СПАРИВАНИЕ ГОМЕОЛОГИЧНЫХ ХРОМОСОМ У ОТДАЛЕННЫХ АЛЛОГАПЛОИДНЫХ ГИБРИДОВ РОДА SOLANUM

CC BY - Creative Commons Attribution 4.0 International

RAI : 20.500.11925/873108

Год создания: 2014
Дата публикации в реестре: 25.08.2017




Описание:
<p align="LEFT">Изучено спаривание хромосом в мейозе уникальных аллогаплоидных гибридов<span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">полученных с ис</span></span>пользованием методов клеточной и хромосомной инженерии<span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">. </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">Использованы две комбинации гиб</span></span>ридов<span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">: 1) </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">между дигаплоидом культурного картофеля </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">Solanum tuberosum </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">(</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">АА</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">-</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">геном</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, 2</span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">n </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 2</span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">x </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 24) </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">и </span></span>дикорастущим видом <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">S. </span></span><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">е</span></span><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">tuberosum </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">(</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">ЕЕ</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, 2</span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">n </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 2</span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">x </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 24); 2) </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">между культурным томатом </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">S. lycopersicum </span></span></em>(LL, 2 <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">n </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 2</span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">x </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 24) </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">и </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">S. etuberosum </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">(</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">ЕЕ</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, 2</span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">n </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 2</span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">x </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">= 24). </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">Хромосомоспецифичные </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">BAC-</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">клоны картофеля </span></span>и пробы дифференциально меченой тотальной ДНК родительских видов позволили идентифицировать конъюгирующие хромосомы и их геномную принадлежность<span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">. </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">У аллогаплоидов </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">S. tuberosum </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">с </span></span>S. etuberosum <span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">геномного состава АЕ обнаружено до </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">7 </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">межгеномных бивалентов на клетку</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">; </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">хиазмы </span></span>формируются в дистальном районе длинного плеча каждой хромосомы набора<span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">; </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">хиазмы в корот</span></span>ком плече имеют хромосомы <span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">3, 6, 11 </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">и </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">12. </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">Для андрогенных регенерантов соматических гибридов </span></span>S. lycopersicum <span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">c </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">S. etuberosum </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">характерен в основном унивалентный мейоз</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">; </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">редкие биваленты </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">(</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">от </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">0 </span></span>до <span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">2 </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">на клетку</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">) </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">образованы гомеологами хромосом </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">4 </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">и </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">6. </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">Обсуждаются перспективы предложенного </span></span>подхода<span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, </span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">основанного на использовании методов соматической гибридизации и </span></span><em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: small;">in vitro </span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">андрогенеза</span></span><span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">, </span></span>для изучения спаривания гомеологичных хромосом и разработки стратегии интрогрессивной гибридизации отдаленных видов растений<span style="font-family: TimesNewRomanPSMT; font-size: small;"><span style="font-family: TimesNewRomanPSMT; font-size: small;">.</span></span></p>

Язык:
rus

Тип:
Научные статьи

Банк знаний:
Научные журналы на платформе Elpub

Источник:
Вавиловский журнал генетики и селекции




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